IL-10 Monoclonal Matched Antibody Pair, PBS Only
Host / Isotype
Capture: Mouse / IgG1
Detection: Mouse / IgG2b
Reactivity
Capture: Human
Detection: Human
Applications
Sandwich ELISA
Range
Sandwich ELISA: 15.6-1000 pg/mL
Validation Data Gallery
Tested Applications
Sandwich ELISA
Recommended dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
MP50035-1 targets IL-10 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.
Range: 15.6-1000 pg/mL (Sandwich ELISA);
Capture antibody: NeutraKine® IL-10 Monoclonal antibody, PBS Only (Capture) 69018-1-PBS (1E4F5). 100 μg. Concentration 1 mgl/ml.
Detection antibody: IL-10 Monoclonal antibody, PBS Only (Detector) 69018-2-PBS (7B8G3). 100 μg. Concentration 1 mgl/ml.
Matched pair protein standard: HZ-1145
Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.
Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)
Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.
| Catalog Number | 69018-1-PBS |
| Tested Reactivity | Human |
| Immunogen | human Humankine IL-10 protein HZ-1145 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interleukin 10 |
| Synonyms | CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, NeutraKine® IL-10, TGIF |
| Gene symbol | IL-10 |
| Gene ID (NCBI) | 3586 |
| Conjugate | Unconjugated |
| Form | Lyophilized Powder |
| Purification Method | Protein A purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |
| Catalog Number | 69018-2-PBS |
| Cited Applications | IHC |
| Tested Reactivity | Human |
| Cited Reactivity | rat |
| Immunogen | human IL-10 protein HZ-1145 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interleukin 10 |
| Synonyms | CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF |
| Gene symbol | IL-10 |
| Gene ID (NCBI) | 3586 |
| Conjugate | Unconjugated |
| Form | Lyophilized Powder |
| Purification Method | Protein A purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |