Phospho-Histone H2A.X (Ser139) Recombinant antibody, PBS Only

Phospho-Histone H2A.X (Ser139) Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5N19

Cat no : 83307-2-PBS

Synonyms

H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x

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Validation Data Gallery

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  WB analysis using 83307-2-RR (same clone as 83307-2-PBS)

  Non-treated Jurkat cells, and staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83307-2-PBS in a different storage buffer formulation.

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  IHC staining of Jurkat using 83307-2-RR (same clone as 83307-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83307-2-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83307-2-PBS targets Phospho-Histone H2A.X (Ser139) in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: H2A histone family, member X
• Calculated Molecular Weight: 15 kDa
• GenBank Accession Number: BC013416
• Gene Symbol: Histone H2A.X
• Gene ID (NCBI): 3014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.