ChromoTek Halo-Trap Magnetic Agarose

The ChromoTek Halo-Trap Magnetic Agarose consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to magnetic agarose beads. Halo-Trap Magnetic Agarose is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

Specificity

Halo-Tag

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic agarose

Cat no : otma

Synonyms

Halo-Trap, Halo, Halo tag



Product Information

The ChromoTek Halo-Trap Magnetic Agarose consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to magnetic agarose beads. Halo-Trap Magnetic Agarose is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

DescriptionImmunoprecipitation of Halo-tagged proteins and their interacting factors with anti-Halo Nanobody conjugated to magnetic agarose beads.

• Halo Trap recognizes Halo fusion proteins that are already bound to chloralkane-based ligands of the Halo-tag, e.g. dyes, biotin, etc.

• Halo-Trap can be used for affinity purification

• Bound Halo-fusion protein can be eluted without protease

• Structure and function are characterized

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetHalo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.
Binding capacity12.5 μg of recombinant Halo-tag per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 M urea, 2 M NaCl, 10 mM DTT,  2 % Nonidet P40 Substitute, 2 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 2 nM
Compatibility with mass spectrometryThe Halo-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2892995
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!