HUWE1 Recombinant antibody

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  WB analysis using 85177-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85177-3-RR (HUWE1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 85177-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HUWE1 Recombinant antibody (85177-3-RR, Clone:242702F10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 85177-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85177-3-RR against Human HUWE1 were performed. The affinity constant is 26.1 nM.

HUWE1 {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 85177-3-RR

Clone No.242702F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

242702F10, ARF binding protein 1, ARF BP1, ARF-binding protein 1, ARF-BP1

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Tested Applications

• Positive WB detected in: A431 cells, NIH/3T3 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85177-3-RR targets HUWE1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HUWE1 fusion protein Ag13763
• Full Name: HECT, UBA and WWE domain containing 1
• Calculated Molecular Weight: 437 aa, 482 kDa
• Observed Molecular Weight: 482 kDa
• GenBank Accession Number: BC002602
• Gene Symbol: HUWE1
• Gene ID (NCBI): 10075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z6Z7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HUWE1 encodes a HECT domain ubiquitin ligase which is a large protein (500 kDa) has attracted considerable interest because several and quite disparate substrates have been assigned to this E3. It has a role in regulating Berg-mann glia differentiation and this ubiquitin ligase orchestrates the programming of the neural progenitors that give rise to neurons and glia in the cerebellum. HUWE1 is essential for proliferation of a subset of tumor cells, and negative regulator of TP53 during the colorectal carcinoma progression through the ubiquitination pathway mediated by the HECT domain (PMID:15567145). HUWE1 plays a critical role in lung cancer and increased HUWE1 expression is significantly associated with worse prognosis which suggest that HUWE1 might be a potential target for lung cancer therapy (PMID: 30026863).