HSP90 Monoclonal antibody, PBS Only

HSP90 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3F11C1

Cat no : 60318-1-PBS

Synonyms

FLJ31884, Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2

---

Validation Data Gallery

• 

  WB analysis using 60318-1-Ig (same clone as 60318-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60318-1-Ig (HSP90 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 60318-1-Ig (same clone as 60318-1-PBS)

  IP result of anti-HSP90 (IP:60318-1-Ig, 5ug; Detection:60318-1-Ig 1:5000) with HepG2 cells lysate 1200 ug. This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 60318-1-Ig (same clone as 60318-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 60318-1-Ig (same clone as 60318-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60318-1-Ig (same clone as 60318-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60318-1-Ig (HSP90 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

---

Product Information

60318-1-PBS targets HSP90 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSP90 fusion protein Ag3826
• Full Name: heat shock protein 90kDa alpha (cytosolic), class A member 1
• Calculated Molecular Weight: 853 aa, 90 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC023006
• Gene Symbol: HSP90
• Gene ID (NCBI): 3320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.