CoraLite® Plus 488-conjugated HSP60 Monoclonal antibody

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  IF Staining of HepG2 using CL488-66041

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66041 (HSP60 antibody) at dilution of 1:50.

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  FC experiment of HepG2 using CL488-66041

  1X10^6 HepG2 cells were intracellularly stained with 1 ug CoraLite® Plus 488 Anti-Human HSP60 (CL488-66041, Clone:2F10E7) (red), or 1 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

HSP60 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-66041

Clone No.2F10E7

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra), RIP

Synonyms

HSPD1, GROEL, EC:5.6.1.7, CPN60, Chaperonin 60

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 1.00 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• RIP: See 1 publications below

Product Information

CL488-66041 targets HSP60 in IF/ICC, FC (Intra), RIP applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSP60 fusion protein Ag7848
• Full Name: heat shock 60kDa protein 1 (chaperonin)
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC003030
• Gene Symbol: HSP60
• Gene ID (NCBI): 3329
• RRID: AB_2883235
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P10809
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Publications

Species	Application	Title
human	RIP	Cancer Lett; circCCNY enhances lenvatinib sensitivity and suppresses immune evasion in hepatocellular carcinoma by serving as a scaffold for SMURF1 mediated HSP60 degradation; Authors - Lei Yang