HSP47/SERPINH1 Monoclonal antibody, PBS Only (Detector)

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  WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67863-1-Ig (same clone as 67863-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67863-1-Ig (same clone as 67863-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67863-1-Ig (same clone as 67863-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 67863-1-Ig (same clone as 67863-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67863-1-Ig (same clone as 67863-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67863-1-Ig (same clone as 67863-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50781-1

  Cytometric bead array standard curve of MP50781-1, HSP47/SERPINH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67863-2-PBS. Detection antibody: 67863-1-PBS. Standard:Ag30999. Range: 0.781-100 ng/mL.

HSP47/SERPINH1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 67863-1-PBS

Clone No.2A8F6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

HSP47, SERPINH1, CBP2, CBP1, AsTP3

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Product Information

67863-1-PBS targets HSP47/SERPINH1 as part of a matched antibody pair:

MP50781-1: 67863-2-PBS capture and 67863-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSP47/SERPINH1 fusion protein Ag30999
• Full Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC014623
• Gene Symbol: HSP47/SERPINH1
• Gene ID (NCBI): 871
• RRID: AB_2918621
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50454
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.