HSP47/SERPINH1 Monoclonal antibody

HSP47/SERPINH1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2A8F6

Reviews (1)

Cat no : 67863-1-Ig

Synonyms

HSP47, SERPINH1, CBP2, CBP1, AsTP3

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Validation Data Gallery

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  WB analysis using 67863-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67863-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67863-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67863-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67863-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 67863-1-Ig

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 67863-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 67863-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67863-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 67863-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, HEK-293T cells, NIH/3T3 cells, LNCaP cells, HeLa cells, Jurkat cells, HEK-293 cells, HSC-T6 cells, 4T1 cells
• Positive IHC detected in: human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

67863-1-Ig targets HSP47/SERPINH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSP47/SERPINH1 fusion protein Ag30999
• Full Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC014623
• Gene Symbol: HSP47
• Gene ID (NCBI): 871
• RRID: AB_2918621
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	J Proteome Res; Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts; Authors - Bridgette Hartley
human	WB,IP,CoIP	Elife; Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum; Authors - Ya-Juan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (03-05-2024)

Worked well in WB in MSCs at 1:2000 in 5% milk

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: MSCs