HPSE Recombinant antibody, PBS Only

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  WB analysis of HeLa using 84562-1-RR (same clone as 84562-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 84562-1-RR (HPSE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84562-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84562-1-RR (same clone as 84562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84562-1-RR (HPSE antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84562-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84562-1-RR (same clone as 84562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84562-1-RR (HPSE antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84562-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84562-1-RR (same clone as 84562-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HPSE Recombinant Antibody (84562-1-RR, Clone:241651E3) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84562-1-PBS in a different storage buffer formulation.

HPSE Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 84562-1-PBS

Clone No.241651E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Heparanase, HEP, Endo-glucoronidase, Endo glucoronidase, EC:3.2.1.166

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Product Information

84562-1-PBS targets HPSE in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HPSE fusion protein Ag21479
• Full Name: heparanase
• Calculated Molecular Weight: 543 aa, 61 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC051321
• Gene Symbol: HPSE
• Gene ID (NCBI): 10855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y251
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.