HMBS Monoclonal antibody, PBS Only

HMBS Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G12H3

Cat no : 67456-1-PBS

Synonyms

HMBS, hydroxymethylbilane synthase, PBG D, PBGD, Porphobilinogen deaminase, Pre uroporphyrinogen synthase, UPS

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Validation Data Gallery

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  WB analysis using 67456-1-Ig (same clone as 67456-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67456-1-Ig (same clone as 67456-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67456-1-Ig (same clone as 67456-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67456-1-Ig (same clone as 67456-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67456-1-Ig (same clone as 67456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67456-1-Ig (same clone as 67456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67456-1-Ig (same clone as 67456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67456-1-Ig (same clone as 67456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67456-1-PBS targets HMBS in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HMBS fusion protein Ag6594
• Full Name: hydroxymethylbilane synthase
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC000520
• Gene Symbol: HMBS
• Gene ID (NCBI): 3145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.