Various lysates were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 67456-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 67456-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 67456-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 67456-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 67456-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67456-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67456-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
MCF-7 cells, HEK-293 cells, HeLa cells, HepG2 cells, Caco-2 cells
Positive IHC detected in
human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:6000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
67456-1-Ig targets HMBS in WB, IHC, ELISA applications and shows reactivity with Human samples.
Various lysates were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 67456-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 67456-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 67456-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 67456-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 67456-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67456-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67456-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
HMBS Monoclonal antibody
Catalog Number
67456-1-Ig
Citations
-
Dilutions
WB : 1:1000-1:6000 IHC : 1:50-1:500
Applications
WB, IHC, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
