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HMBS Monoclonal antibody

HMBS Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G12H3

Cat no : 67456-1-Ig

Synonyms

HMBS, hydroxymethylbilane synthase, PBG D, PBGD, Porphobilinogen deaminase, Pre uroporphyrinogen synthase, UPS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HMBS Monoclonal antibody (67456-1-Ig)

    WB analysis using 67456-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using HMBS Monoclonal antibody (67456-1-Ig)

    WB analysis of HepG2 using 67456-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HMBS Monoclonal antibody (67456-1-Ig)

    WB analysis of HEK-293 using 67456-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HMBS Monoclonal antibody (67456-1-Ig)

    WB analysis of HeLa using 67456-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HMBS Monoclonal antibody (67456-1-Ig)

    IHC staining of human breast cancer using 67456-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HMBS Monoclonal antibody (67456-1-Ig)

    IHC staining of human breast cancer using 67456-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HMBS Monoclonal antibody (67456-1-Ig)

    IHC staining of human liver cancer using 67456-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HMBS Monoclonal antibody (67456-1-Ig)

    IHC staining of human liver cancer using 67456-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, HeLa cells, HepG2 cells, Caco-2 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67456-1-Ig targets HMBS in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HMBS fusion protein Ag6594
Full Name hydroxymethylbilane synthase
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-42 kDa
GenBank Accession NumberBC000520
Gene Symbol HMBS
Gene ID (NCBI) 3145
RRIDAB_2882688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HMBS antibody 67456-1-IgDownload protocol
IHC protocol for HMBS antibody 67456-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols