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Cardinal Red™-conjugated HIF-1 alpha Polyclonal antibody

HIF-1 alpha Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Cardinal Red™ Fluorescent Dye

Cat no : CR-20960

Synonyms

ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF1 ALPHA, HIF-1 alpha, HIF1A, Member of PAS protein 1, MOP1, PASD8



Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CR-20960 is HIF-1 alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIF-1 alpha fusion protein Ag15198
Full Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Calculated Molecular Weight 826 aa, 93 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC012527
Gene Symbol HIF1A
Gene ID (NCBI) 3091
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths592 nm / 611 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

Protocols

Product Specific Protocols
FC protocol for Cardinal Red™ HIF-1 alpha antibody CR-20960Download protocol
Standard Protocols
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