HIF-1 alpha antibody (80933-1-PBS)

Filter: AllWBIHCIF/ICCAffinity

WB analysis of HeLa using 80933-1-RR (same clone as 80933-1-PBS)
Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 80933-1-RR (HIF1a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 80933-1-RR (same clone as 80933-1-PBS)
WB result of HIF-1 alpha antibody (80933-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
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WB result of HIF-1 alpha antibody (80933-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 80933-1-RR (same clone as 80933-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 80933-1-RR (HIF-1 alpha antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid cancer using 80933-1-RR (same clone as 80933-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80933-1-RR (HIF1a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid cancer using 80933-1-RR (same clone as 80933-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80933-1-RR (HIF1a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80933-1-RR (same clone as 80933-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80933-1-RR (HIF-1 alpha antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 80933-1-RR (same clone as 80933-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80933-1-RR (HIF-1 alpha antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 80933-1-RR (same clone as 80933-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594+phalloidin (red). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 80933-1-RR (same clone as 80933-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 80933-1-RR (same clone as 80933-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 80933-1-RR
Biolayer interferometry (BLl) kinetic assays of 80933-1-RR against Human HIF-1 alpha were performed. The affinity constant is below 1 pM.

Featured Product
KD/KO Validated

HIF-1 alpha Recombinant antibody, PBS Only

HIF-1 alpha Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 80933-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HIF1A, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1, ARNT-interacting protein

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

80933-1-PBS targets HIF-1 alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	HIF-1 alpha fusion protein Ag15198 Predict reactive species
Full Name	hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Calculated Molecular Weight	826 aa, 93 kDa
Observed Molecular Weight	120 kDa
GenBank Accession Number	BC012527
Gene Symbol	HIF1A
Gene ID (NCBI)	3091
RRID	AB_2918921
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q16665
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, PMID: 7539918).