HIF1a Recombinant antibody

HIF1a Recombinant Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2K1

Cat no : 80933-1-RR

Synonyms

ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF1 ALPHA, HIF1A, Member of PAS protein 1, MOP1, PASD8



Tested Applications

Positive WB detected inCobalt Chloride treated HeLa cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCobalt Chloride treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

80933-1-RR targets HIF1a in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HIF1a fusion protein Ag15198
Full Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Calculated molecular weight 826 aa, 93 kDa
Observed molecular weight120 kDa
GenBank accession numberBC012527
Gene symbol HIF1A
Gene ID (NCBI) 3091
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, PMID: 7539918).

Protocols

Product Specific Protocols
WB protocol for HIF1a antibody 80933-1-RRDownload protocol
IHC protocol for HIF1a antibody 80933-1-RRDownload protocol
IF protocol for HIF1a antibody 80933-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols