HEXIM1 Recombinant antibody

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  WB analysis using 85028-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85028-1-RR (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85028-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEXIM1 antibody (85028-1-RR, Clone: 242587A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

HEXIM1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85028-1-RR

Clone No.242587A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1, CLP 1

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, A431 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85028-1-RR targets HEXIM1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HEXIM1 fusion protein Ag8144
• Full Name: hexamethylene bis-acetamide inducible 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC006460
• Gene Symbol: HEXIM1
• Gene ID (NCBI): 10614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O94992
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).