HEXIM1 Monoclonal antibody, PBS Only

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  WB analysis of Caco-2 using 66311-1-Ig (same clone as 66311-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66311-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66311-1-Ig (same clone as 66311-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66311-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66311-1-Ig (same clone as 66311-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66311-1-PBS in a different storage buffer formulation.

HEXIM1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66311-1-PBS

Clone No.2C8A2

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1, CLP 1

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Product Information

66311-1-PBS targets HEXIM1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: HEXIM1 fusion protein Ag8019
• Full Name: hexamethylene bis-acetamide inducible 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC006460
• Gene Symbol: HEXIM1
• Gene ID (NCBI): 10614
• RRID: AB_2881693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• UNIPROT ID: O94992
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).