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HEXIM1 Monoclonal antibody, PBS Only

HEXIM1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C8A2

Cat no : 66311-1-PBS

Synonyms

Cardiac lineage protein 1, CLP1, EDG1, HEXIM1, HIS1, MAQ1, Menage a quatre protein 1, Protein HEXIM1

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using HEXIM1 Monoclonal antibody (66311-1-Ig)

    WB analysis of human testis using 66311-1-Ig (same clone as 66311-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66311-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using HEXIM1 Monoclonal antibody (66311-1-Ig)

    WB analysis of MCF-7 using 66311-1-Ig (same clone as 66311-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66311-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66311-1-PBS targets HEXIM1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen HEXIM1 fusion protein Ag8019
Full Name hexamethylene bis-acetamide inducible 1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC006460
Gene Symbol HEXIM1
Gene ID (NCBI) 10614
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).