HEXIM1 Monoclonal antibody

HEXIM1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C8A2

Cat no : 66311-1-Ig

Synonyms

Cardiac lineage protein 1, CLP1, EDG1, HEXIM1, HIS1, MAQ1, Menage a quatre protein 1, Protein HEXIM1

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Validation Data Gallery

• 

  WB analysis of human testis using 66311-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66311-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human testis tissue, MCF-7 cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66311-1-Ig targets HEXIM1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: HEXIM1 fusion protein Ag8019
• Full Name: hexamethylene bis-acetamide inducible 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC006460
• Gene Symbol: HEXIM1
• Gene ID (NCBI): 10614
• RRID: AB_2881693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).