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HEXIM1 Monoclonal antibody

HEXIM1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C8A2

Cat no : 66311-1-Ig

Synonyms

Cardiac lineage protein 1, CLP1, EDG1, HEXIM1, HIS1, MAQ1, Menage a quatre protein 1, Protein HEXIM1

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using HEXIM1 Monoclonal antibody (66311-1-Ig)

    WB analysis of human testis using 66311-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using HEXIM1 Monoclonal antibody (66311-1-Ig)

    WB analysis of MCF-7 using 66311-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman testis tissue, MCF-7 cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66311-1-Ig targets HEXIM1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen HEXIM1 fusion protein Ag8019
Full Name hexamethylene bis-acetamide inducible 1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC006460
Gene Symbol HEXIM1
Gene ID (NCBI) 10614
RRIDAB_2881693
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).

Protocols

Product Specific Protocols
WB protocol for HEXIM1 antibody 66311-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols