CoraLite®594-conjugated HE4 Monoclonal antibody

HE4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5B2D9

Cat no : CL594-66557

Synonyms

dJ461P17.6, HE4, WAP5, WFDC2

---

Validation Data Gallery

• 

  IF Staining of SKOV-3 using CL594-66557

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite®®594 HE4 antibody (CL594-66557, Clone: 5B2D9 ) at dilution of 1:200.

---

Tested Applications

• Positive IF/ICC detected in: SKOV-3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66557 targets HE4 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HE4 fusion protein Ag5936
• Full Name: WAP four-disulfide core domain 2
• Calculated Molecular Weight: 13 kDa
• GenBank Accession Number: BC046106
• Gene Symbol: HE4
• Gene ID (NCBI): 10406
• RRID: AB_2920004
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.