CoraLite®594-conjugated HE4 Monoclonal antibody

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  IF Staining of SKOV-3 using CL594-66557

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using CoraLite®594 HE4 antibody (CL594-66557, Clone: 5B2D9 ) at dilution of 1:200.

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  IF Staining of SKOV-3 using CL594-66557

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite®®594 HE4 antibody (CL594-66557, Clone: 5B2D9 ) at dilution of 1:200.

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  FC experiment of OVCAR-3 using CL594-66557

  1x10^6 OVCAR-3 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated HE4 Monoclonal antibody (CL594-66557, Clone:5B2D9)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of SKOV-3 using CL594-66557

  1x10^6 SKOV-3 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated HE4 Monoclonal antibody (CL594-66557, Clone:5B2D9)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

HE4 Monoclonal Antibody for IF/ICC

Cat No. CL594-66557

Clone No.5B2D9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

WFDC2, WAP5, WAP four-disulfide core domain protein 2, WAP four disulfide core domain protein 2, Major epididymis-specific protein E4

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Tested Applications

• Positive IF/ICC detected in: SKOV-3 cells
• Positive FC (Intra) detected in: SKOV-3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66557 targets HE4 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HE4 fusion protein Ag5936
• Full Name: WAP four-disulfide core domain 2
• Calculated Molecular Weight: 13 kDa
• GenBank Accession Number: BC046106
• Gene Symbol: HE4
• Gene ID (NCBI): 10406
• RRID: AB_2920004
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q14508
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.