HE4 Monoclonal antibody
HE4 Monoclonal Antibody for IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
5B2D9
Cat no : 66557-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SKOV-3 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:1000-1:5000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66557-1-Ig targets HE4 in IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | HE4 fusion protein Ag5936 |
Full Name | WAP four-disulfide core domain 2 |
Calculated Molecular Weight | 13 kDa |
GenBank Accession Number | BC046106 |
Gene Symbol | HE4 |
Gene ID (NCBI) | 10406 |
RRID | AB_2881918 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.
Protocols
Product Specific Protocols | |
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IHC protocol for HE4 antibody 66557-1-Ig | Download protocol |
IF protocol for HE4 antibody 66557-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |