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HE4 Monoclonal antibody, PBS Only

HE4 Monoclonal Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5B2D9

Cat no : 66557-1-PBS

Synonyms

HE4, WAP5, WFDC2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HE4 Monoclonal antibody (66557-1-Ig)

    IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HE4 Monoclonal antibody (66557-1-Ig)

    IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using HE4 Monoclonal antibody (66557-1-Ig)

    IF Staining of SKOV-3 using 66557-1-Ig (same clone as 66557-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using HE4 antibody (66557-1-Ig, Clone: 5B2D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66557-1-PBS targets HE4 in IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HE4 fusion protein Ag5936
Full Name WAP four-disulfide core domain 2
Calculated Molecular Weight 13 kDa
GenBank Accession NumberBC046106
Gene Symbol WFDC2
Gene ID (NCBI) 10406
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.