HA Tag Monoclonal antibody, PBS Only

HA Tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1F5C6

Cat no : 66006-2-PBS

Synonyms

YPYDVPDYA

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Validation Data Gallery

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  WB analysis of HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66006-2-Ig (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Ag19172 using 66006-2-Ig (same clone as 66006-2-PBS)

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.1μg/mL. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Ag19172 using 66006-2-Ig (same clone as 66006-2-PBS)

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.05μg/mL. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Ag19172 using 66006-2-Ig (same clone as 66006-2-PBS)

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.025μg/mL. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Ag19172 using 66006-2-Ig (same clone as 66006-2-PBS)

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.0125μg/mL. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

• 

  IP experiment of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

  IP result of anti-HA Tag (IP:66006-2-Ig, 5ug; Detection:66006-2-Ig 1:2000) with Transfected HEK-293 cells lysate 1600 ug. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

• 

  IF Staining of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (66006-2-Ig, Clone: 1F5C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

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Product Information

66006-2-PBS targets HA Tag in WB, IF/ICC, IP, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: HA Tag
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.