FITC Plus Anti-Human Granzyme B Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using FITC-98070

  1x10^6 human PBMCs were intracellularly stained with APC Anti-Human CD8a, and 5 ul FITC Plus Anti-Human Granzyme B Rabbit RecAb (FITC-98070, Clone: 240761G4) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using FITC-98070

  1x10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human Granzyme B Rabbit RecAb (FITC-98070, Clone: 240761G4) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Granzyme B Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. FITC-98070

Clone No.240761G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

GZMB, 240761G4, Cathepsin G-like 1, CCPI, CGL 1

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98070 targets Granzyme B in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Calculated Molecular Weight: 247 aa, 28 kDa
• GenBank Accession Number: BC030195
• Gene Symbol: Granzyme B
• Gene ID (NCBI): 3002
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10144
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

GZMB (Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces lamin B degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full-length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32 kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.