Anti-Human Granzyme B Rabbit Recombinant Antibody

• 

  FC experiment of human PBMCs using 98070-1-RR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD8a (OKT8) (APC-65135, Clone: OKT8), and 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using 98070-1-RR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) or isotype control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG. Cells were stained with APC Anti-Human CD8a before fixing. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using 98070-1-RR

  1x10^6 human PBMCs were stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) (red), or Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98070-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98070-1-RR against Human Granzyme B were performed. The affinity constant is 0.144 nM.

Granzyme B Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98070-1-RR

Clone No.240761G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

GZMB, CSP B, CGL1, CGL 1, CCPI

---

Tested Applications

• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98070-1-RR targets Granzyme B in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Calculated Molecular Weight: 247 aa, 28 kDa
• GenBank Accession Number: BC030195
• Gene Symbol: Granzyme B
• Gene ID (NCBI): 3002
• RRID: AB_3672217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10144
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

GZMB (Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces lamin B degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full-length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32 kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.