Anti-Human Granzyme B Rabbit Recombinant Antibody, PBS Only

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  FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD8a (OKT8) (APC-65135, Clone: OKT8), and 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98070-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) or isotype control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG. Cells were stained with APC Anti-Human CD8a before fixing. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98070-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

  1x10^6 human PBMCs were stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) (red), or Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98070-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98070-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98070-1-RR against Human Granzyme B were performed. The affinity constant is 0.144 nM.

Granzyme B Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98070-1-PBS

Clone No.240761G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

GZMB, CSP B, CGL1, CGL 1, CCPI

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Product Information

98070-1-PBS targets Granzyme B in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Calculated Molecular Weight: 247 aa, 28 kDa
• GenBank Accession Number: BC030195
• Gene Symbol: Granzyme B
• Gene ID (NCBI): 3002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10144
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GZMB (Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces lamin B degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full-length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32 kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.