CoraLite® Plus 488 Anti-Mouse GM-CSF Rabbit Recombinant Antibody

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  FC experiment of C57BL/6 CD3 T cells using CL488-98014

  1x10^6 unstimulated (left) or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) (right) C57BL/6 CD3 T cells were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse Gm-csf Rabbit Recombinant Antibody (CL488-98014, Clone:230286E11) and APC Anti-Mouse CD3. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

GM-CSF Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. CL488-98014

Clone No.230286E11

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Csf2, Gm CSf, Csfgm, CSF 2, Colony Stimulating Factor 2

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Tested Applications

• Positive FC (Intra) detected in: PMA and ionomycin stimulated C57BL/6 CD3 T cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-98014 targets GM-CSF in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: colony stimulating factor 2 (granulocyte-macrophage)
• GenBank Accession Number: NM-009969
• Gene Symbol: GM-CSF
• Gene ID (NCBI): 12981
• RRID: AB_3673362
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01587
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Gm-csf, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. Gm-csf was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. Gm-csf is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. Gm-csf has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.