Anti-Mouse Gm-csf Rabbit Recombinant Antibody

Gm-csf Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230286E11

Cat no : 98014-1-RR

Synonyms

Csf2, Gm CSf, Csfgm, CSF 2, Colony Stimulating Factor 2

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Validation Data Gallery

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  FC experiment of C57BL/6 CD3 T cells using 98014-1-RR

  1x10^6 unstimulated or PMA and ionomycin in presence of protein transport inhibitors stimulated C57BL/6 CD3 T cells were intracellularly stained with 0.25 ug Anti-Mouse Gm-csf (98014-1-RR, Clone: 230286E11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.5 ug FITC Plus Anti-Mouse CD3 (17A2) (FITC-65077, Clone: 17A2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98014-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98014-1-RR against Human Gm-csf were performed. The affinity constant is 2.25 nM.

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Tested Applications

• Positive FC (Intra) detected in: PMA and ionomycin stimulated C57BL/6 CD3 T cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98014-1-RR targets Gm-csf in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: colony stimulating factor 2 (granulocyte-macrophage)
• GenBank Accession Number: NM-009969
• Gene Symbol: GM-CSF
• Gene ID (NCBI): 12981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Gm-csf, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. Gm-csf was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. Gm-csf is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. Gm-csf has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.