Galectin 3 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Galectin 3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30450-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 30450-1-AP (same clone as 30450-1-PBS)

  WB result of Galectin 3 antibody (30450-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin 3 transfected HeLa cells. This data was developed using the same antibody clone with 30450-1-PBS in a different storage buffer formulation.

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  WB analysis using 30450-1-AP (same clone as 30450-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30450-1-AP (Galectin 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30450-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 30450-1-AP (same clone as 30450-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30450-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 30450-1-AP (same clone as 30450-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30450-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 30450-1-AP (same clone as 30450-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30450-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 30450-1-AP (same clone as 30450-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30450-1-PBS in a different storage buffer formulation.

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Product Information

30450-1-PBS targets Galectin 3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: lectin, galactoside-binding, soluble, 3
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 29-31 kDa
• GenBank Accession Number: BC001120
• Gene Symbol: Galectin-3
• Gene ID (NCBI): 3958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).