Galectin 3 Polyclonal antibody

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  WB analysis of HeLa using 30450-1-AP

  WB result of Galectin 3 antibody (30450-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin 3 transfected HeLa cells.

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  WB analysis using 30450-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30450-1-AP (Galectin 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 30450-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 30450-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 30450-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 30450-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Galectin 3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30450-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Galectin-3, GAL3, Gal 3, CBP35, CBP 35

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, mouse colon tissue, rat colon tissue
• Positive IHC detected in: human thyroid cancer tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30450-1-AP targets Galectin 3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: lectin, galactoside-binding, soluble, 3
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 29-31 kDa
• GenBank Accession Number: BC001120
• Gene Symbol: Galectin-3
• Gene ID (NCBI): 3958
• RRID: AB_3086318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17931
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).