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  • Featured Product
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Galectin 3 Polyclonal antibody

Galectin 3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30450-1-AP

Synonyms

35 kDa lectin, CBP 35, CBP35, Gal 3, GAL3, Galactose specific lectin 3, Galactoside binding protein, GALBP, Galectin 3, GALIG, IgE binding protein, L 31, Laminin binding protein, Lectin L 29, LGALS2, LGALS3, Mac 2 antigen, MAC2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Galectin 3 Polyclonal antibody (30450-1-AP)

    WB analysis of HeLa using 30450-1-AP

    WB result of Galectin 3 antibody (30450-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin 3 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using Galectin 3 Polyclonal antibody (30450-1-AP)

    WB analysis using 30450-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30450-1-AP (Galectin 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Galectin 3 Polyclonal antibody (30450-1-AP)

    IHC staining of human thyroid cancer using 30450-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Galectin 3 Polyclonal antibody (30450-1-AP)

    IHC staining of human thyroid cancer using 30450-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Galectin 3 Polyclonal antibody (30450-1-AP)

    IHC staining of mouse colon using 30450-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Galectin 3 Polyclonal antibody (30450-1-AP)

    IHC staining of mouse colon using 30450-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA431 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, mouse colon tissue, rat colon tissue
Positive IHC detected inhuman thyroid cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30450-1-AP targets Galectin 3 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein
Full Name lectin, galactoside-binding, soluble, 3
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 29-31 kDa
GenBank Accession NumberBC001120
Gene Symbol Galectin-3
Gene ID (NCBI) 3958
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

Protocols

Product Specific Protocols
WB protocol for Galectin 3 antibody 30450-1-APDownload protocol
IHC protocol for Galectin 3 antibody 30450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols