CoraLite® Plus 488-conjugated Galectin-1 Monoclonal antibody
Galectin-1 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3G10D2
Cat no : CL488-60223
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | human colon cancer tissue, U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
Product Information
The immunogen of CL488-60223 is Galectin-1 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Galectin-1 fusion protein Ag2432 |
Full Name | lectin, galactoside-binding, soluble, 1 |
Calculated Molecular Weight | 15 kDa |
Observed Molecular Weight | 13 kDa |
GenBank Accession Number | BC020675 |
Gene Symbol | Galectin-1 |
Gene ID (NCBI) | 3956 |
RRID | AB_2919238 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Galectin-1 antibody CL488-60223 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Margarita (Verified Customer) (06-06-2023) |
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