CoraLite® Plus 488-conjugated Galectin-1 Monoclonal antibody

• Featured Product
• KD/KO Validated

Galectin-1 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

2

CloneNo.

3G10D2

Reviews (1)

Cat no : CL488-60223

Synonyms

Gal 1, Beta-galactoside-binding lectin L-14-I, 3G10D2, 14 kDa lectin, 14 kDa laminin-binding protein

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Validation Data Gallery

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  IF Staining of human colon cancer using CL488-60223

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 Galectin-1 antibody (CL488-60223, Clone: 3G10D2 ) at dilution of 1:800.

• 

  IF Staining of U2OS using CL488-60223

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 Galectin-1 antibody (CL488-60223, Clone: 3G10D2 ) at dilution of 1:200.

• 

  FC experiment of U2OS using CL488-60223

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Galectin-1 Monoclonal antibody (CL488-60223, Clone:3G10D2)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: human colon cancer tissue, U2OS cells
• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-60223 targets Galectin-1 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Galectin-1 fusion protein Ag2432
• Full Name: lectin, galactoside-binding, soluble, 1
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC020675
• Gene Symbol: Galectin-1
• Gene ID (NCBI): 3956
• RRID: AB_2919238
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

Publications

Species	Application	Title
human	IF	Sci Rep; The role of GCNT1 mediated O-glycosylation in aggressive prostate cancer; Authors - Kirsty Hodgson
		mBio; Herpes simplex virus type 1 modifies the protein composition of extracellular vesicles to promote neurite outgrowth and neuroinfection; Authors - Guorong Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Margarita (Verified Customer) (06-06-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: PC3 cells

CL Plus 488 Galectin-1 Antibody Immunofluorescence validation (1:200 dilution) in PC3 cells (Cat no:CL488-60223)