Galectin-1 Monoclonal antibody, PBS Only

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  WB analysis using 60223-1-Ig (same clone as 60223-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60223-1-Ig (Galectin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60223-1-Ig (same clone as 60223-1-PBS)

  WB result of Galectin-1 antibody (60223-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin-1 transfected HepG2 cells. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60223-1-Ig (same clone as 60223-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60223-1-Ig (Galectin-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 60223-1-Ig (same clone as 60223-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60223-1-Ig (Galectin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 60223-1-Ig (same clone as 60223-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 60223-1-Ig (GAL1,LGALS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60223-1-Ig (same clone as 60223-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 60223-1-Ig (same clone as 60223-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

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  IF Staining of Saos-2 using 60223-1-Ig (same clone as 60223-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 60223-1-Ig (same clone as 60223-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Galectin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 60223-1-PBS

Clone No.3G10D2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

3G10D2, Beta-galactoside-binding lectin L-14-I, Gal 1, GAL1, Gal-1

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Product Information

60223-1-PBS targets Galectin-1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2432

  Product name: Recombinant human Galectin 1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-135 aa of BC020675

  Sequence: MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD

• Full Name: lectin, galactoside-binding, soluble, 1
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC020675
• Gene Symbol: Galectin-1
• Gene ID (NCBI): 3956
• RRID: AB_11182608
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P09382
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).