CoraLite® Plus 488-conjugated GYPC Polyclonal antibody

GYPC Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18147

Synonyms

CD236, CD236R, GE, GLPC, Glycoconnectin, Glycophorin C, Glycophorin D, Glycoprotein beta, GPC, GPD, GYPC, GYPD, PAS 2, Sialoglycoprotein D

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Validation Data Gallery

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  IF Staining of K-562 using CL488-18147

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite® Plus 488 GYPC antibody (CL488-18147) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18147 targets GYPC in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GYPC fusion protein Ag12825
• Full Name: glycophorin C (Gerbich blood group)
• Calculated Molecular Weight: 128 aa, 14 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC106051
• Gene Symbol: GYPC
• Gene ID (NCBI): 2995
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glycophorin-C, encoded by the GYPC gene, is a minor sialoglycoprotein in human erythrocyte membranes. Glycophorin-C interacts with protein 4.1R (EPB41) and plays an important role in regulating the mechanical stability of red cells. Glycophorin C acts as the receptor for the Plasmodium falciparum erythrocyte binding ligand PfEBP-2 (baebl). Glycophorin-D, also encoded by the GYPC gene, is now known as a variant of Glycophorin-C. This antibody is raised against the full-length protein of human Glycophorin-C. The apparent molecular weight of Glycophorin-C is larger than the calculated molecular weight due to glycosylation.