GSS Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

GSS Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F12G1

Cat no : 67598-1-PBS

Synonyms

Glutathione synthase, glutathione synthetase, GSH S, GSH synthetase, GSHS, GSS

---

Validation Data Gallery

• 

  WB analysis using 67598-1-Ig (same clone as 67598-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67598-1-Ig (same clone as 67598-1-PBS)

  WB result of GSS antibody (67598-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSS transfected HEK-293 cells. This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67598-1-Ig (same clone as 67598-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67598-1-Ig (same clone as 67598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67598-1-Ig (same clone as 67598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67598-1-Ig (same clone as 67598-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSS antibody (67598-1-Ig, Clone: 1F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67598-1-PBS targets GSS in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, pig samples.

• Tested Reactivity: Human, Mouse, Rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GSS fusion protein Ag8354
• Full Name: glutathione synthetase
• Calculated Molecular Weight: 474 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC007927
• Gene Symbol: GSS
• Gene ID (NCBI): 2937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.