Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of HEK-293 using 67598-1-Ig
WB result of GSS antibody (67598-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSS transfected HEK-293 cells.
WB result of GSS antibody (67598-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSS transfected HEK-293 cells.
WB analysis using 67598-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IHC staining of human liver cancer using 67598-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67598-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 67598-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSS antibody (67598-1-Ig, Clone: 1F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSS antibody (67598-1-Ig, Clone: 1F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
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CHO cells, rat liver tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected in
human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of HEK-293 using 67598-1-Ig
WB result of GSS antibody (67598-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSS transfected HEK-293 cells.
WB analysis using 67598-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IHC Figures
IHC staining of human liver cancer using 67598-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67598-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 67598-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSS antibody (67598-1-Ig, Clone: 1F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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