GSS Monoclonal antibody

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• KD/KO Validated

GSS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig, hamster and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1F12G1

Cat no : 67598-1-Ig

Synonyms

Glutathione synthase, glutathione synthetase, GSH S, GSH synthetase, GSHS, GSS

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Validation Data Gallery

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  WB analysis using 67598-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HEK-293 using 67598-1-Ig

  WB result of GSS antibody (67598-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSS transfected HEK-293 cells.

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  WB analysis using 67598-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of human liver cancer using 67598-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67598-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67598-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSS antibody (67598-1-Ig, Clone: 1F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: CHO cells, rat liver tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67598-1-Ig targets GSS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, pig, hamster samples.

• Tested Reactivity: Human, Mouse, Rat, pig, hamster
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GSS fusion protein Ag8354
• Full Name: glutathione synthetase
• Calculated Molecular Weight: 474 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC007927
• Gene Symbol: GSS
• Gene ID (NCBI): 2937
• RRID: AB_2882804
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	World J Gastroenterol; Bile acids inhibit ferroptosis sensitivity through activating farnesoid X receptor in gastric cancer cells; Authors - Chu-Xuan Liu
human	WB	J Biol Chem; Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity; Authors - Xiaohan Jin