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GSS Monoclonal antibody

GSS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig, hamster and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F12G1

Cat no : 67598-1-Ig

Synonyms

Glutathione synthase, glutathione synthetase, GSH S, GSH synthetase, GSHS, GSS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSS Monoclonal antibody (67598-1-Ig)

    WB analysis using 67598-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using GSS Monoclonal antibody (67598-1-Ig)

    WB analysis of HEK-293 using 67598-1-Ig

    WB result of GSS antibody (67598-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSS transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using GSS Monoclonal antibody (67598-1-Ig)

    WB analysis using 67598-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSS Monoclonal antibody (67598-1-Ig)

    IHC staining of human liver cancer using 67598-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSS Monoclonal antibody (67598-1-Ig)

    IHC staining of human liver cancer using 67598-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSS Monoclonal antibody (67598-1-Ig)

    IF Staining of HeLa using 67598-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSS antibody (67598-1-Ig, Clone: 1F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inCHO cells, rat liver tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67598-1-Ig targets GSS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, pig, hamster samples.

Tested Reactivity Human, Mouse, Rat, pig, hamster
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSS fusion protein Ag8354
Full Name glutathione synthetase
Calculated Molecular Weight 474 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC007927
Gene Symbol GSS
Gene ID (NCBI) 2937
RRIDAB_2882804
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GSS antibody 67598-1-IgDownload protocol
IHC protocol for GSS antibody 67598-1-IgDownload protocol
IF protocol for GSS antibody 67598-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

World J Gastroenterol

Bile acids inhibit ferroptosis sensitivity through activating farnesoid X receptor in gastric cancer cells

Authors - Chu-Xuan Liu
View Article
humanWB

J Biol Chem

Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity

Authors - Xiaohan Jin
View Article