CoraLite®594-conjugated GRB2 Monoclonal antibody

• Featured Product
• KD/KO Validated

GRB2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C5D11

Cat no : CL594-66880

Synonyms

Adapter protein GRB2, ASH, EGFRBP GRB2, GRB2, Grb3 3, MST084, MSTP084, Protein Ash, SH2/SH3 adapter GRB2

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66880

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 GRB2 antibody (CL594-66880, Clone: 1C5D11 ) at dilution of 1:200.

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  IF Staining of HeLa using CL594-66880

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 GRB2 antibody (CL594-66880, Clone: 1C5D11 ) at dilution of 1:200, CL488-phalloidin (green).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66880 targets GRB2 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GRB2 fusion protein Ag0395
• Full Name: growth factor receptor-bound protein 2
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC000631
• Gene Symbol: GRB2
• Gene ID (NCBI): 2885
• RRID: AB_2920051
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-β protein precursor (AβPP). Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.