GRB2 Monoclonal antibody

• Featured Product
• KD/KO Validated

GRB2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1C5D11

Cat no : 66880-1-Ig

Synonyms

Adapter protein GRB2, ASH, EGFRBP GRB2, GRB2, Grb3 3, MST084, MSTP084, Protein Ash, SH2/SH3 adapter GRB2

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Validation Data Gallery

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  WB analysis using 66880-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66880-1-Ig (GRB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66880-1-Ig

  WB result of GRB2 antibody (66880-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRB2 transfected HEK-293 cells.

• 

  WB analysis using 66880-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66880-1-Ig (GRB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66880-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66880-1-Ig (GRB2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66880-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66880-1-Ig (GRB2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Raji cells, Daudi cells, Ramos cells, Karpas-422 cells, Karpas-299 cells, MOLT-4 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

66880-1-Ig targets GRB2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GRB2 fusion protein Ag0395
• Full Name: growth factor receptor-bound protein 2
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC000631
• Gene Symbol: GRB2
• Gene ID (NCBI): 2885
• RRID: AB_2882212
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-β protein precursor (AβPP). Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.

Publications

Species	Application	Title
rat	WB	FASEB J; Low-magnitude vibration induces osteogenic differentiation of bone marrow mesenchymal stem cells via miR-378a-3p/Grb2 pathway to promote bone formation in a rat model of age-related bone loss.; Authors - Xiaoqin Yu ; KD Validated
human	WB	Sci Adv; HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis; Authors - Qiwei Jiang