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CoraLite® Plus 488-conjugated GPX4 Recombinant antibody

GPX4 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230168A8

Cat no : CL488-82949

Synonyms

GSHPx 4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated GPX4 Recombinant ant (CL488-82949)

    IF Staining of HepG2 using CL488-82949

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 GPX4 antibody (CL488-82949, Clone: 230168A8 ) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82949 targets GPX4 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GPX4 fusion protein Ag5809
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Calculated Molecular Weight 22 kDa
GenBank Accession NumberBC022071
Gene Symbol GPX4
Gene ID (NCBI) 2879
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GPX4 antibody CL488-82949Download protocol
Standard Protocols
Click here to view our Standard Protocols