GPX4 Recombinant antibody

GPX4 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230168A8

Cat no : 82949-1-RR

Synonyms

GSHPx 4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

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Validation Data Gallery

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  IHC staining of mouse testis using 82949-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82949-1-RR (GPX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 82949-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82949-1-RR (GPX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 82949-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GPX4 antibody (82949-1-RR, Clone: 230168A8 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 82949-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GPX4 Recombinant antibody (82949-1-RR, Clone:230168A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82949-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82949-1-RR against Human GCLM were performed. The affinity constant is 1 pM.

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Tested Applications

• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82949-1-RR targets GPX4 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GPX4 fusion protein Ag5809
• Full Name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Calculated Molecular Weight: 22 kDa
• GenBank Accession Number: BC022071
• Gene Symbol: GPX4
• Gene ID (NCBI): 2879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.