CoraLite® Plus 488-conjugated GPX4 Polyclonal antibody

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  IF Staining of HeLa using CL488-30388

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 GPX4 antibody (CL488-30388) at dilution of 1:200.

GPX4 Polyclonal Antibody for IF/ICC

Cat No. CL488-30388

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

Synonyms

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-30388 targets GPX4 in applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPX4 fusion protein Ag30650
• Full Name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC021567
• Gene Symbol: GPX4
• Gene ID (NCBI): 2879
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36969
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.