GPX4 Recombinant antibody, PBS Only (Detector)

GPX4 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230168A8

Cat no : 82949-1-PBS

Synonyms

GSHPx 4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00049-3

  Sandwich ELISA standard curve of MP00049-3, Human GPX4 Recombinant Matched Antibody Pair - PBS only. 82949-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5809. 82949-1-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL

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  Sample test of MP00049-3

  HEK-293 cell lysate was measured. The human GPX4 concentration of detected samples was determined to be 8.1 ng/mL (based on a 1.2 mg/mL extract load).

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  IHC staining of mouse testis using 82949-1-RR (same clone as 82949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82949-1-RR (GPX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82949-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82949-1-RR (same clone as 82949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82949-1-RR (GPX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82949-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82949-1-RR (same clone as 82949-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GPX4 antibody (82949-1-RR, Clone: 230168A8 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82949-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82949-1-RR (same clone as 82949-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GPX4 Recombinant antibody (82949-1-RR, Clone:230168A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82949-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00049-1

  Cytometric bead array standard curve of MP00049-1, GPX4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82949-3-PBS. Detection antibody: 82949-1-PBS. Standard: Ag5809. Range: 2.5-20 ng/mL.

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  Cytometric bead array standard curve of MP00049-3

  Cytometric bead array standard curve of MP00049-3, GPX4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82949-4-PBS. Detection antibody: 82949-1-PBS. Standard: Ag5809. Range: 2.5-20 ng/mL.

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  Affinity and Kinetic Characterization of 82949-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82949-1-RR against Human GCLM were performed. The affinity constant is 1 pM.

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Product Information

82949-1-PBS targets GPX4 as part of a matched antibody pair:

MP00049-1: 82949-3-PBS capture and 82949-1-PBS detection (validated in Cytometric bead array)

MP00049-3: 82949-4-PBS capture and 82949-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GPX4 fusion protein Ag5809
• Full Name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Calculated Molecular Weight: 22 kDa
• GenBank Accession Number: BC022071
• Gene Symbol: GPX4
• Gene ID (NCBI): 2879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.