GPSM1 Monoclonal antibody, PBS Only

GPSM1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A7A2

Cat no : 68233-1-PBS

Synonyms

AGS3, DKFZp727I051, GPSM1

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Validation Data Gallery

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  WB analysis using 68233-1-Ig (same clone as 68233-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68233-1-Ig (GPSM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68233-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 68233-1-Ig (same clone as 68233-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using GPSM1 antibody (68233-1-Ig, Clone: 3A7A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68233-1-PBS in a different storage buffer formulation.

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Product Information

68233-1-PBS targets GPSM1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GPSM1 fusion protein Ag18773
• Full Name: G-protein signaling modulator 1 (AGS3-like, C. elegans)
• Calculated Molecular Weight: 652 aa, 72 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC017353
• Gene Symbol: GPSM1
• Gene ID (NCBI): 26086
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GPSM1, also named as AGS3, is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/function, macroautophagy and metabolism. It contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. It has been shown that AGS3 could regulate the orientation of the mitotic spindle, cAMP production, membrane protein transport, and asymmetric cell division by binding preferentially to inactive Gai/o subunits complexed with guanine dinucleotide phosphate (GDP) at multiple G-protein regulatory or GoLoco motif repeats. It also plays an important anti-apoptotic role through enhancing the phosphorylation of cyclic AMP response element-binding protein (p-CREB).