GPSM1 Monoclonal antibody

GPSM1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3A7A2

Cat no : 68233-1-Ig

Synonyms

AGS3, DKFZp727I051, GPSM1



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68233-1-Ig targets GPSM1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPSM1 fusion protein Ag18773
Full Name G-protein signaling modulator 1 (AGS3-like, C. elegans)
Calculated Molecular Weight 652 aa, 72 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC017353
Gene Symbol GPSM1
Gene ID (NCBI) 26086
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPSM1, also named as AGS3, is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/function, macroautophagy and metabolism. It contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. It has been shown that AGS3 could regulate the orientation of the mitotic spindle, cAMP production, membrane protein transport, and asymmetric cell division by binding preferentially to inactive Gai/o subunits complexed with guanine dinucleotide phosphate (GDP) at multiple G-protein regulatory or GoLoco motif repeats. It also plays an important anti-apoptotic role through enhancing the phosphorylation of cyclic AMP response element-binding protein (p-CREB).

Protocols

Product Specific Protocols
WB protocol for GPSM1 antibody 68233-1-IgDownload protocol
IF protocol for GPSM1 antibody 68233-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols