Biotin Plus-conjugated GPM6A Polyclonal antibody

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  IHC staining of mouse brain using Biotin-15044

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-15044 (GPM6A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPM6A Polyclonal Antibody for IHC

Cat No. Biotin-15044

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Synonyms

Neuronal membrane glycoprotein M6-a, M6A, GPM6

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-15044 targets GPM6A in IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPM6A fusion protein Ag6915
• Full Name: glycoprotein M6A
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC022528
• Gene Symbol: GPM6A
• Gene ID (NCBI): 2823
• Conjugate: Biotin Plus
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51674
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glycoprotein M6A (GPM6A, or M6A) is known as a multi-pass transmembrane protein and an abundant cell surface protein on neurons in the central nervous system (CNS). M6A belongs to the myelin proteolipid protein family and is involved in neuronal differentiation, including differentiation and migration of neuronal stem cell. M6A is identified as a retinal membrane protein that is strongly expressed at embryonic stages. In mouse adult retina, M6A plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation via regulation of MAPK and Src signaling pathways.