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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated GP73/GOLPH2 Monoclonal antibody

GP73/GOLPH2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D1F1

Cat no : CL594-66331

Synonyms

GOLM1, GOLPH2, GP73, golgi protein 73kD, Golgi phosphoprotein 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated GP73/GOLPH2 Monoclonal ant (CL594-66331)

    IF Staining of HeLa using CL594-66331

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 GP73/GOLPH2 antibody (CL594-66331, Clone: 3D1F1 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated GP73/GOLPH2 Monoclonal ant (CL594-66331)

    IF Staining of HepG2 using CL594-66331

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66331 (GP73/GOLPH2 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated GP73/GOLPH2 Monoclonal ant (CL594-66331)

    FC experiment of HeLa using CL594-66331

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GP73/GOLPH2 (CL594-66331, Clone:3D1F1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66331 targets GP73/GOLPH2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GP73/GOLPH2 fusion protein Ag7207
Full Name golgi membrane protein 1
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC001740
Gene Symbol GP73
Gene ID (NCBI) 51280
RRIDAB_2883560
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.

Protocols

Product Specific Protocols
IF protocol for CL594 GP73/GOLPH2 antibody CL594-66331Download protocol
FC protocol for CL594 GP73/GOLPH2 antibody CL594-66331Download protocol
Standard Protocols
Click here to view our Standard Protocols