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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated GORASP2 Polyclonal antibody

GORASP2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10598

Synonyms

GRS2, GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated GORASP2 Polyclonal antibod (CL594-10598)

    IF Staining of HepG2 using CL594-10598

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 GORASP2 antibody (CL594-10598) at dilution of 1:600, CL488-Phalloidin (green).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated GORASP2 Polyclonal antibod (CL594-10598)

    FC experiment of HepG2 using CL594-10598

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GORASP2 (CL594-10598) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10598 targets GORASP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GORASP2 fusion protein Ag0950
Full Name golgi reassembly stacking protein 2, 55kDa
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC007770
Gene Symbol GORASP2
Gene ID (NCBI) 26003
RRIDAB_2919740
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Protocols

Product Specific Protocols
IF protocol for CL594 GORASP2 antibody CL594-10598Download protocol
FC protocol for CL594 GORASP2 antibody CL594-10598Download protocol
Standard Protocols
Click here to view our Standard Protocols