GOLGA5 Recombinant antibody, PBS Only (Capture/Detector)

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  WB analysis using 83839-2-RR (same clone as 83839-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83839-2-RR (GOLGA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83839-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83839-2-RR (same clone as 83839-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83839-2-RR (GOLGA5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83839-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00813-2

  Cytometric bead array standard curve of MP00813-2, GOLGA5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83839-2-PBS. Detection antibody: 83839-2-PBS. Standard: Ag35031. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00813-3

  Cytometric bead array standard curve of MP00813-3, GOLGA5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83839-5-PBS. Detection antibody: 83839-2-PBS. Standard: Ag35031. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83839-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83839-2-RR against Human GOLGA5 were performed. The affinity constant is 5.79 nM.

GOLGA5 {ptg:Brand} Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 83839-2-PBS

Clone No.240893F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

GOLIM5, Golgin-84, Golgin subfamily A member 5, Golgin 84, Cell proliferation-inducing gene 31 protein

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Product Information

83839-2-PBS targets GOLGA5 as part of a matched antibody pair:

MP00813-2: 83839-2-PBS capture and 83839-2-PBS detection (validated in Cytometric bead array)

MP00813-3: 83839-5-PBS capture and 83839-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GOLGA5 fusion protein Ag35031
• Full Name: golgi autoantigen, golgin subfamily a, 5
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 80-95 kDa
• GenBank Accession Number: BC023021
• Gene Symbol: GOLGA5
• Gene ID (NCBI): 9950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TBA6
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

GOLGA5 (Golgin subfamily A member 5) maintains Golgi structure, and stimulates the formation of Golgi stacks and ribbons. Also, it is involved in intra-Golgi retrograde transport (PMID: 12538640; 15718469).