GOLGA5 Recombinant antibody

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  WB analysis using 83839-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83839-2-RR (GOLGA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 83839-2-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83839-2-RR (GOLGA5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83839-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83839-2-RR against Human GOLGA5 were performed. The affinity constant is 5.79 nM.

GOLGA5 Recombinant Antibody for WB, IHC, ELISA

Cat No. 83839-2-RR

Clone No.240893F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

GOLIM5, Golgin-84, Golgin subfamily A member 5, Golgin 84, Cell proliferation-inducing gene 31 protein

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83839-2-RR targets GOLGA5 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GOLGA5 fusion protein Ag35031
• Full Name: golgi autoantigen, golgin subfamily a, 5
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 80-95 kDa
• GenBank Accession Number: BC023021
• Gene Symbol: GOLGA5
• Gene ID (NCBI): 9950
• RRID: AB_3671422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TBA6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOLGA5 (Golgin subfamily A member 5) maintains Golgi structure, and stimulates the formation of Golgi stacks and ribbons. Also, it is involved in intra-Golgi retrograde transport (PMID: 12538640; 15718469).